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DNA-dependent ATPase activities of wild-type and mutant UvrD protein
| Enzyme | Sp act (U/μg)a | kcat (s−1) | Km (μM) |
|---|---|---|---|
| UvrD+ | 656 ± 29 | 81 ± 4 | 55 ± 5 |
| UvrD303 | 888 ± 71 | 129 ± 11 | 59 ± 8 |
The unit for specific activity is defined as the amount of protein hydrolyzing 1 nmol of ATP in 10 min at 37°C.
