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Purification of FKBP from M. thermolithotrophicus DSM2095
| Purification step | Total vol (ml) | Protein concn (mg/ml) | Total activity (U) | Sp act (U/mg) | Recovery (%) |
|---|---|---|---|---|---|
| Crude extract | 300 | 0.86 | 1,040 | 4.03 | 100 |
| Butyl-Sepharose FF | 40 | 0.23 | 439 | 47.7 | 42 |
| Superose 12HR | 15 | 0.15 | 143 | 63.5 | 14 |
| Mono Q | 3.7 | 0.041 | 79.4 | 550 | 7.7 |
| TSK gel Ether-5PW | 1.5 | 0.027 | 37.4 | 800 | 3.6 |
