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. 1998 Jan;180(2):388–394. doi: 10.1128/jb.180.2.388-394.1998

TABLE 2.

Catalytic efficiencies of MTFK and other FKBPs to N-suc-Ala-Xaa-Pro-Phe-pNA

Xaa kcat/Km (μM−1 s−1)a of:
FKBP
CyP
MTFKb Legionella MIPc E. coli trigger factord Bovine FKBPe E. coli CyP 18cyf Bovine CyPe
Ala 0.20 0.05 0.16 0.05 67.4 3.18
Leu 0.35 1.10 0.43 0.64 23.4 2.70
Leu/Alag 1.75 22.0 2.69 12.8 0.35 0.71
a

The values were determined by the chymotrypsin-coupled assay (see Materials and Methods) at 10°C. 

b

This study (15°C for MTFK). 

c

Reference 21

d

Reference 34

e

Reference 13

f

Reference 7 (15°C for E. coli CyP 18cy). 

g

The ratio of kcat/Km for N-suc-A-L-P-F-pNA to that for N-suc-A-A-P-F-pNA.