Skip to main content
. 2023 Nov 30;14:7916. doi: 10.1038/s41467-023-43402-z

Fig. 7. DLPC induces iWAT browning via lipid peroxidation-mediated p38 activation.

Fig. 7

a FACS profiles of Bodipy C11-stained iWAT-derived adipocytes treated with vehicle or 0.25 mM DLPC for 30 min in the presence or absence of the lipid peroxidation inhibitor, liproxstatin-1 (Lip-1). b Heatmap showing redox proteomics results from iWAT-derived adipocytes treated with vehicle or 1 mM DLPC for 30 min in the presence or absence of Lip-1. c Relative mRNA levels of Ucp1 in iWAT-derived primary adipocytes treated with vehicle or various doses of DLPC (0.25, 0.5, 1 mM) for 12 h in the presence or absence of the lipid peroxidation inhibitors, Lip-1 or Fer-1. For DLPC group, p = 0.0025 (Vehicle vs. 0.5 mM), p < 0.0001 (Vehicle vs. 1 mM); For DLPC + Fer-1 group, p < 0.0001 (Vehicle vs. 0.25 mM), p < 0.0001 (Vehicle vs. 0.5 mM), p < 0.0001 (Vehicle vs. 1 mM); For DLPC + Lip-1 group, p = 0.0345 (Vehicle vs. 0.25 mM), p = 0.0014 (Vehicle vs. 0.5 mM), p = 0.0002 (Vehicle vs. 1 mM). Data are representative of three independent experiments. d Pathway enrichment from phospho-proteomics analysis of iWAT-derived adipocytes treated with vehicle or 1 mM of DLPC for 30 min in the presence or absence of Lip-1. e Network showing the proposed involvement of p38 and the MAPK cascade in the DLPC-mediated signaling of the iWAT-derived adipocytes described in (d). f–h Representative Western blots showing p38 phosphorylation in iWAT-derived adipocytes treated with vehicle or various doses of DLPC (0.25, 0.5 mM) for 12 h (f), or 1 mM DLPC for the indicated durations (5 min, 15 min, 30 min, 1 h, 6 h) (g), or 1 mM DLPC for 12 h in the presence or absence of Lip-1 or BIRB796 (an inhibitor of p38) (h). i Relative mRNA levels of Ucp1 in iWAT-derived adipocytes treated as described in (h), as determined by RT-qPCR. p = 0.0228 (Vehicle vs. DLPC), p = 0.0245 (DLPC vs. DLPC+Lip-1), p = 0.0352 (DLPC vs. DLPC + BIRB796). Data are representative of three independent experiments. j, k Representative Western blots showing p38 phosphorylation in iWAT from C57BL/6j mice i.p. administered with vehicle or various doses of DLPC (50, 100, 200 mg/kg) for 30 min (j) or 50 mg/kg DLPC for 30 min in the presence or absence of Lip-1 (k). β-Tubulin served as an equal loading control. Data are presented as mean ± SD. Significance was assessed by one-way ANOVA (i) or two-way ANOVA (c). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to vehicle control group. Source data are provided as a Source Data file.