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. 2023 Nov 1;55(11):2390–2401. doi: 10.1038/s12276-023-01108-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b qRT‒PCR (a) and Western blot (b) results showing the mRNA and protein levels of m6A regulators in HBx-oe and NC cells. c Dual-luciferase assay results indicating the activity of the RBM15 gene promoter in HBx-oe and NC cells. d Western blot results showing the knockdown efficiency of si-RBM15 in HCC cells. e, f MeRIP-qPCR (e) and qRT‒PCR (f) results showing the m6A level and expression of cFAM210A in si-RBM15 and si-NC cells. g Western blot results showing the knockdown efficiency of si-RBM15 and overexpression efficiency of the HBx-oe vector in HepG2 cells. h, i MeRIP-qPCR (h) and qRT‒PCR (i) results in HepG2 cells showing that the effects of si-RBM15 were abolished by overexpressing HBx. For (a), (f) and (i), ACTB was used as an endogenous control. For (a), (c), (e), (f), (h) and (i), Student’s t test was used. NC negative control, HBx-oe HBx-overexpressing, ns not significant. *P < 0.05; **P < 0.01; ***P < 0.001.