Fig. 5. Sorafenib-induced and DDX5-repressed genes enriched in Wnt/β-catenin signaling.
A Venn diagram of common genes between SOR-induced and DDX5-repressed genes. B GSEA plot showing enrichment of genes more highly expressed in SOR vs. DMSO treated WT HepAD38 cells, and repressed by DDX5. C Top 10-most enriched KEGG pathways associated with genes induced by SOR and repressed by DDX5. D qRT PCR of indicated Wnt/β-catenin signaling genes using RNA from HepAD38 cells treated with sorafenib (7.5 µM) for 3 days. Data are mean ± SEM, n = 3. *p < 0.05, **p < 0.01 by unpaired t-test. E Kaplan–Meier survival plots for DVL1 expression of SOR treated patients with HCC. Samples are from TCGA. F–H qRT-PCR of DVL1 mRNA and immunoblots of DVL1 protein, using total RNA or lysates, respectively, isolated from: F WT Huh7 cells transfected with siCtlr or siDDX5, G WT and DDX5OE Huh7 cells, grown as described in Fig. 4A, and H WT and DDX5KO Huh7 cells, ±SOR (10 µM) for 24 h, as indicated. qRT-PCR data are expressed as mean ± SEM from n = 3. *p < 0.05, **p < 0.01 by unpaired t-test. A representative DVL1 immunoblot is shown n = 3. Actin is used as a loading control.