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Selective and mutagenic primers
| Primer | Sequencea |
|---|---|
| Selective | CACTTTGACATCGACCCAAGTACC |
| Mutagenic primers | |
| R443L | GCCAAGGCTCTCGTCATCTCC |
| P451S | GCCGGCGTGTCCGTGTCGCCG |
| P454S | CCCGTGTCGTCGTCGAGCCCC |
| S456N | TCGCCGTCGAACCCCAAGAAA |
| P457S | CCGTCGAGCTCCAAGAAAACC |
| K458M | TCGAGCCCCATGAAAACCATG |
| K459I | AGCCCCAAGATAACCATGACT |
Substitutions in the selective and the mutagenic primers are underlined.
