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. 2023 Sep 28;55(11):1679–1685. doi: 10.1007/s00726-023-03340-y

Table 2.

Primers for qRT-PCR

Gene Primer Sense (5’–3’) Primer Antisense (5’–3’) PCR Product (bp) Ta (°C)
Col1a1 TGATGGTGCTACTGGTGCTG CCTCGCTTTCCTTCCTCTCC 437 60
Col4a1 CTACGTGCAAGGCAATGAACG GCAGAACAGGAAGGGCATTGT 93 60
Eln TTCCCCGCAGTTACCTTTCC ACGTTCCCAGGCTTCACTCC 175 60
Fbn GTGGTGTGGTCTACTCTGTGG TCTGGTCGGCATCATAGTTCTG 434 60
GAPDH GGCTGAGAACGGGAAGCTTGT CCAGCATCGCCCCACTTGAT 90 60