TABLE 1.
Purification of dienelactone hydrolase of Pseudomonas sp. strain B13
| Purification step | Vol (ml) | Amt of protein (mg) | Total activity (U)a | Sp. act. (U/g)a | Recov- ery (%) | Purifi- cation (fold) |
|---|---|---|---|---|---|---|
| Cell extract | 5 | 146 | 37.9 (0.35) | 260 (2.4) | 100 | 1 |
| Mono Q eluate | 2.5 | 1.25 | 25.0 (0.25) | 20,000 (200) | 66 | 77 |
| Phenyl-Sepharose eluate | 0.27 | 0.28 | 7.3 (0.067) | 26,500 (240) | 19 | 102 |
| Superose 6 eluate | 1.5 | 0.10 | 5.8 (0.058) | 58,800 (580) | 15 | 226 |
a
Values are given for cis-dienelactone as the substrate. Values in parentheses are the corresponding values for protoanemonin as the substrate.