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. 2023 Nov 30;14:7886. doi: 10.1038/s41467-023-43531-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Schematic representation of gene arrangement on ptSYN24 neochromosome. b Fitness of ySYN24 in different growth conditions. WT, BY4741. c The doubling time of ySYN24 (n = 3) in YPD. WT, BY4742 (n = 3). Source data are provided as a Source Data file. The data are presented as the mean and SD. d The cell morphology of ySYN24. WT, BY4742. Scale bars, 5 μm. Images are representative of at least three independent samples. e FACS analysis after propidine iodide staining on asynchronous cells. WT, BY4742. f Differentially expressed genes on ptSYN24 in ySYN24. The nine essential TUs, whose counterpart in ptWT10U-10KO were also differentially expressed, were labeled in brown. g Functional enrichment analysis of the differentially expressed genes in ySYN24. The differential genes were mapped to a global similarity network annotated using SAFE. The colors represent different function domains. Triangles with white rims indicate genes located in chrXIIL.