TABLE 2.
ATPase hydrolytic activities and proton transport rates in plasma membrane vesicles from U. maydis sporidiaa
Strain and treatment | VH+ (AU min−1 mg of protein−1) | Hydrolytic activity (μmol of Pi min−1 mg−1) | Coupling index (AU [10−2] μmol of Pi−1) | Latency (%) |
---|---|---|---|---|
IMI 103761 | ||||
Et-C | 391.6 ± 46.4 | 2.313 ± 0.059 | 2.92 ± 0.26 | 57.5 ± 6.1 |
Tri-T | 872.8 ± 38.5 AC | 4.147 ± 0.475 AC | 2.70 ± 0.77 | 65.5 ± 1.7 C |
Fen-T | 489.2 ± 78.5 D | 3.339 ± 0.295 A | 1.80 ± 0.31 AD | 72.9 ± 1.5 AD |
WT | 457.8 ± 71.5 | 2.499 ± 0.061 | 3.00 ± 0.36 | 44.8 ± 8.3 |
A14 (none) | 596.4 ± 65.0 | 1.761 ± 0.300 B | 4.20 ± 0.65 | 4.4 ± 4.4 B |
P51 (none) | 171.2 ± 17.5 B | 2.893 ± 0.087 B | 0.71 ± 0.17 B | 53.3 ± 2.9 |
Data are means ± SEs of three independent experiments. Letters indicate significant differences as evaluated by the least-significant-difference test with respect to Et-C-treated sporidia (A), control WT sporidia (B), A14 (C), and P51 (D).