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. 2023 Oct 27;64(12):100465. doi: 10.1016/j.jlr.2023.100465

Fig. 4.

Fig. 4

TMEM241 performs as an UDP-GlcNAc transporter to regulate NPC2 lysosomal targeting. A, B: Western blot analysis of lysosomal hydrolases: cathepsin D (CTSD) and cathepsin L (CTSL) in the whole-cell lysate and culture medium of HeLa/TMEM241 KO cells and WT cells. On the left, different forms of Cathepsin D are denoted as follows: “p” for precursor, “i” for intermediate, and “m” for mature heavy chain. WCL, whole-cell lysate. C: The relative activity of lysosomal enzyme β-hexosaminidase in whole-cell lysates and culture media of control and TMEM241 KO cells. Results are normalized to the protein amounts. Data are represented as mean ± SD (n = 3). ∗∗∗P < 0.001, Student’s t test. D: Diagrammatic sketch of M6P-dependent lysosomal targeting of the luminal proteins. E: Immunoblot analysis of HeLa, HeLa/S1P KO, HeLa/SLC35A3 KO, and HeLa/TMEM241 KO cells using the anti-M6P antibody and actin antibody. F: Immunoblot analysis of WT cells and the TMEM241 KO cells stably expressing TMEM241-3×FLAG or 3×FLAG-SLC35A3. M6P, mannose-6-phosphate; NPC, Niemann-Pick disease type C; UDP-GlcNAc, UDP-N-acetylglucosamine; WCL, whole-cell lysate.