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Pulsed-field gel stained with ethidium bromide showing separation of chromosome-sized DNA of L.tarentolae and the amplicon bands present in the JBP1 mutants. The arrow is pointing at the region containing the linear amplicons. Lanes M: Saccharomyces cerevisiae marker; 1, TarIIWT; 2, JBP1 KO HYG; 3, JBP1 KO PUR; 4, JBP1 KO HYG:KO PUR; 5, JBP1 KO NEO; 6, JBP1 KO HYG:KO NEO; 7, JBP1 KO PUR:KO HYG; and 8, JBP1 KO HYG:KO PUR:KO NEO.
