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Guanidinium-induced unfolding of 4 μM DraSSB (circles) and 3.5 μM EcoSSB (triangles) in 0.3 M NaCl and 20 mM KPi. Apparent molar masses (cf. Materials and Methods) were determined from sedimentation equilibrium centrifugation at 20°C and 14 000 r.p.m. Note the different axes.
