TABLE 1.

Kinetic parameters of wild-type and mutant NfsA and Frp

Enzyme	Substrate	Km (μM)	Vmax (μmol/min/mg)	Vmax/Km
Wild-type NfsA	NADPHa,b	11.0	85	7.7
	NADPHc	0.8	1	1.5
	Nitrofurazonea,d	5.5	45	8.1
	FMNd	2.5	1	0.4
NfsA-97-99G	NADPHb	17.8	46	2.6
	NADPHc	2.9	46	16
	Nitrofurazoned	27.1	65	2.4
	FMNd	29.3	50	1.7
Frpe	NADPHb	12.4	145	12
	NADPHc	7.9	25	3.1
	Nitrofurazoned	19.5	142	7.3
	FMNd	25.8	23	0.9

^aZenno et al. (13). 

^bKinetic parameters obtained by using nitrofurazone as an electron acceptor. 

^cKinetic parameters obtained by using FMN as an electron acceptor. 

^dKinetic parameters obtained by using NADPH as an electron donor. 

^eZenno et al. (12).