Table 2.

Oligonucleotides used for constructing mutated target sites of siNPY

NPY08G-F	5′-GATCTCAATGAGAGAGAGCACAGAAAACGGGCC-3′
NPY08G-R	5′-CGTTTTCTGTGCTCTCTCTCATTGA-3′
NPY08T-F	5′-GATCTCAATGAGAGATAGCACAGAAAACGGGCC-3′
NPY08T-R	5′-CGTTTTCTGTGCTATCTCTCATTGA-3′
NPY08C-F	5′-GATCTCAATGAGAGACAGCACAGAAAACGGGCC-3′
NPY08C-R	5′-CGTTTTCTGTGCTGTCTCTCATTGA-3′
NPY13A-F	5′-GATCTCAATGAGAGAAAGCAAAGAAAACGGGCC-3′
NPY13A-R	5′-CGTTTTCTTTGCTTTCTCTCATTGA-3′
NPY13G-F	5′-GATCTCAATGAGAGAAAGCAGAGAAAACGGGCC-3′
NPY13G-R	5′-CGTTTTCTCTGCTTTCTCTCATTGA-3′
NPY13T-F	5′-GATCTCAATGAGAGAAAGCATAGAAAACGGGCC-3′
NPY13T-R	5′-CGTTTTCTATGCTTTCTCTCATTGA-3′
NPY16G-F	5′-GATCTCAATGAGAGAAAGCACAGGAAACGGGCC-3′
NPY16G-R	5′-CGTTTCCTGTGCTTTCTCTCATTGA-3′
NPY16C-F	5′-GATCTCAATGAGAGAAAGCACAGCAAACGGGCC-3′
NPY16C-R	5′-CGTTTGCTGTGCTTTCTCTCATTGA-3′
NPY16T-F	5′-GATCTCAATGAGAGAAAGCACAGTAAACGGGCC-3′
NPY16T-R	5′-CGTTTACTGTGCTTTCTCTCATTGA-3′

Degenerate nucleotides were shown according to the IUB convention (B = C, G or T; D = A, G or T; H = A, C or T; and V = A, C or G). The regions encoding the target sites were underlined.