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. 2023 Nov 20;150(22):dev202305. doi: 10.1242/dev.202305

Fig. 2.

Fig. 2.

Dendrites of interneurons. (A) Box plots of mean dendritic length (top row) and mean dendritic segment numbers (bottom row) of all interneurons after DIV 11-15 ChR2-eYFP stimulation with 0.05 Hz, 0.5 Hz and 2.5 Hz, each with pulse duration of 70 ms and 140 ms (in shades of blue). The number above each box is the number of neurons analyzed. P-values determined with ANOVA on ranks corrected for multiple testing versus the handling control (in red). (B) Box plots of mean dendritic length (left) and mean dendritic segment numbers (right) of BCs and non-BCs. Handling control in red, stimulation with 70 ms and 140 ms conditions pooled in blue. The number above the boxes is the number of neurons analyzed. P-values determined with Mann–Whitney rank sum test versus the handling control. (C) Sketches depicting the measured dendrites (black). The AcD of AcD cells (middle) was compared with the average of all other dendrites of the AcD cell (right), and with the average of the dendrites of non-AcD cells (left). (D) Length (top row) and segments (bottom row) of the three groups of dendrites for BCs (left column) and non-BCs (right column). The number of cells is given below every box. The number of somatic axon cells is higher than the number of AcD cells. A total of 128 BC and 158 non-BC was analyzed. Of the latter, three cells had two axons from two dendrites, and these AcDs were included. First, we compared control with stimulated cells; Mann–Whitney rank sum test; P-values are the numbers above the lines. Second, we compared the AcD versus the regular dendrites (control cells in red, 0.5 Hz stimulated cells in blue); P-values determined with ANOVA on ranks (AoR). Significant differences in black or color, all other P-values in gray lettering. (E) Percentage of BCs and non-BCs in which the AcD is the longest, second longest or one of the remaining dendrites. Box plots show median (middle bars) and first to third interquartile ranges (boxes); whiskers indicate the 10th and 90th percentile range; dots indicate outliers.