Fig. 4.

The lateral and basal patterning defects in DAAM; frl double-mutant eyes. (A-F) Confocal z-sections of a wild-type (A,C,E) and a DAAM^Ex4; frl⁵⁹ double-mutant eye (B,D,F) at 48 h APF stained for actin (A,B,E,F) and chaoptin (C,D). Although the apical pattern was largely normal in the formin mutant (compare A with B), the lateral section revealed the fused ommatidial clusters (C,D), and in the basal section, the irregularly spaced axonal exit sites are evident (arrows in F). (G-I) Sagittal pupal eye sections of a wild-type (G) and two DAAM^Ex4; frl⁵⁹ double-mutant (H,I) eyes stained for actin. Dense staining of the rhabdomeres marks the main axis of the facets and indicates the basal axonal exit sites, which are regularly spaced in the wild-type eye (G). Note the distorted rhabdomere orientation and irregular spacing of the exit sites (red arrows) in the formin double mutants (H,I). Images are representative of ten animals per genotype. Scale bars: 10 μm.