Fig. 5.

Rescue of the ommatidia fusion with wild-type and actin polymerization-incompetent formins. (A,B) Quantification of the ommatidia fusion phenotypes in pupal eyes of the genotypes indicated. IOC-specific (54C-Gal4-driven) (A) and eye-specific (GMR-Gal4-driven) (B) expression of the wild-type FRL or DAAM-PB isoform was able to rescue the ommatidium fusion phenotype of the formin double mutants to a large extent, although GMR-Gal4 provided a somewhat better rescue than 54C-Gal4. Conversely, the mutant forms impaired in actin polymerization (FRL-I773A and DAAM-PB I732A) did not rescue at all; instead, they enhanced the fusion phenotype, presumably by a dominant-negative effect also exhibited upon their expression in a wild-type background. ‘n’ indicates the number of ommatidia counted. Data are representative of 10-14 animals per genotype.