Fig. 7.

DAAM^Ex4; frl⁵⁹ double-mutant eyes exhibit reduced cortical actin levels in IOCs. (A-D) Phalloidin staining of DAAM^Ex4; frl⁵⁹ double-mutant eyes revealed reduced actin levels along the cortical cell membranes of the IOCs in the apical (A,B) and lateral sections (C,D) of the eye compared with those in wild-type controls (quantified in G). (E,F) Although a similar actin intensity analysis was not feasible in the endfeet region because the cell shape in the double mutants is highly irregular (F), the basal cortical actin signal clearly appeared more diffuse and weaker in the formin mutants than in the wild type (E). Scale bars: 10 µm. (G) Quantification of actin intensity along the cortical membrane of the IOCs on apical and lateral confocal z-sections in wild-type (wt) and formin mutant eyes. Data show the mean±s.d. and are representative of ten animals per genotype. ****P<0.0001 (two-tailed, unpaired Mann–Whitney test).