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. 2023 Dec;64(12):1956–1964. doi: 10.2967/jnumed.123.265418

FIGURE 4.

FIGURE 4.

Gd-NP uptake and subcellular localization. (A) Cellular uptake was measured in SK-OV-3-luc cells by inductively coupled plasma mass spectrometry after 18 h of incubation with Gd-NPs, 10 mg/mL. Results are mean ± SD of 3 independent experiments performed in triplicate. (B) Cytoplasmic localization of Gd-NP Alexa Fluor 488 (Life Technologies Corp.) (left) and trastuzumab (right) by immunofluorescence analysis. (C) Colocalization of Gd-NP Alexa Fluor 488 with lysosomes (Lysotracker red DND-99; Molecular Probes, Inc.) but not with mitochondria (Mitotracker red CMXRos; Molecular Probes, Inc.). ICP-MS = inductively coupled plasma mass spectrometry.