Skip to main content
[Preprint]. 2023 Nov 21:2023.11.21.568134. [Version 1] doi: 10.1101/2023.11.21.568134

FIGURE 2: AC is required for sound-triggered delayed reward prediction.

FIGURE 2:

A. Top: An illustration of the cannula implanted in AC for muscimol infusion. Right: Histological verification of bilateral muscimol infusion into AC. The brain slice acquired from an example animal is overlaid with the corresponding coronal section from the Allen Mouse Brain Atlas (see Methods). The cannula tracks are indicated by the green dotted line on the brain slice and the site of muscimol infusion is seen in orange. Markers on the right indicate the depth at which muscimol was infused in the left and right hemispheres. Scale bar: 1000μm. Bottom: Behavioral timeline for mice that underwent training on the 1.5s Delay task. B. Average peri-sound lick rate response curves (solid line denotes mean, shaded area represents SEM across trials) of an example animal trained to predict reward at 1.5s sound-reward interval when infused with PBS (orange) and muscimol (MUS, black) in AC for rewarded trials (left) and catch trials (right). Shaded pink region represents the sound period. Solid and dotted orange lines represent when reward was given in rewarded trials and expected in catch trials. ***p=0.0007 (Wilcoxon rank-sum test) denotes the significant difference in predictive licking compared to baseline for catch trials on PBS and MUS conditions. C. Average peri-sound lick rate response curves (solid line denotes mean, shaded area represents SEM across trials) of an example animal trained on the No-Delay task when infused with PBS (light blue) and muscimol (MUS, black) in AC for rewarded trials (left) and catch trials (right). Shaded pink region represents the 1.5s long sound period. Solid and dotted blue lines represent when reward was given in rewarded trials and expected in catch trials. n.s. (p=0.939, Wilcoxon rank-sum test) denotes the not significant difference in predictive licking between catch trials on PBS and MUS in the No-Delay task. D. Significant difference in average log predictive licking ratio (log PLR) between No-Delay (N = 8) and 1.5s Delay (N = 8) cohorts (***p =0.000156, Wilcoxon rank-sum test). Error bars represent mean ± SEM across animals. Open circles indicate the PLR for each animal in the No-Delay (blue) and 1.5s Delay cohorts.