Table 2. RNA-seq comparison of selected markers of T cell activation.
Pairwise differential expression analyses between antigen-unexposed and post 12-hour co-culture samples of either YT-rCTL.a3a or a3a TCR-T cell format effectors. Values represent DESeq2 Normalized Counts. Group size for each condition was n=1.
| Gene | YT-rCTL.a3a | YT-rCTL.a3a + MAGEA3 sAPC | a3a TCR-T | a3a TCR-T + MAGEA3 sAPC |
|---|---|---|---|---|
|
TNFRSF9 (CD137 or 4-1BB) |
1,328 | 5,428 | 458 | 45,533 |
| CD69 | 332 | 399 | 4,715 | 7,851 |
|
NR4A1 (Nur77) |
763 | 1,152 | 145 | 6,974 |
| NFATC1 | 178 | 160 | 639 | 1,535 |
| IL2 | 52 | 31 | 38 | 22,986 |
| IL3 | 376 | 312 | 335 | 191,076 |
| XCL1 | 1,328 | 1,168 | 4,111 | 668,187 |
| IFNG | 7,162 | 6,497 | 4,911 | 1,666,865 |
| CCL4 | 77 | 473 | 1,196 | 25,597 |