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[Preprint]. 2023 Nov 20:2023.11.20.567784. [Version 1] doi: 10.1101/2023.11.20.567784

Figure 2. ZAP mutated at positively selected sites show differential antiviral activity against SINV.

Figure 2.

(A, C) Western blot of (A) ZAPS or (C) ZAPL wild-type (WT) or positive selection mutants inducible ZAP KO 293T cell lysates. (B, D) (B) ZAPS or (D) ZAPL WT or mutant ZAP KO 293T cells were induced for ZAP 24 hours before infection with SINV Toto1101/Luc at a multiplicity of infection (MOI) of 0.01 plaque forming units (PFU)/cell and harvested at 24 hours post-infection (h.p.i.) for luciferase assay by measuring relative luciferase units (RLU). Data are combined from two independent experiments; error bars indicate standard deviation. 1μg/mL dox is used to induce ZAP expression. Asterisks indicate statistically significant differences as compared to the -dox condition for each cell line (Two-way ANOVA and Tukey’s multiple comparisons test: *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001).