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[Preprint]. 2023 Nov 22:2023.11.21.568166. [Version 1] doi: 10.1101/2023.11.21.568166

Fig. 2. Transcriptomic analysis identifies changes at the neuromuscular junction (NMJ) in in DmCFL KD muscles.

Fig. 2.

(A) Wandering third instar larvae with muscle mCherry RNAi (control) or tsr RNAi (DmCFL KD) were dissected, RNA extracted, and the RNA sequenced. Right: The volcano plot shows expression profiles in control and DmCFL KD with significantly upregulated (red) and downregulated (blue) genes marked. N = 3 replicates, each with n = 7-10 larvae per genotype. Diagram made using Biorender. (B) Gene ontology (GO) biological processes (BP) pathways identified as decreased significantly in DmCFL KD compared to control (false discovery rate 0.05, minimum fold change −2). (C) GO BP pathways identified as increased significantly in DmCFL KD compared to control (false discovery rate 0.05, minimum fold change 2). (D) Fold change of select genes related to the motor neuron and NMJ function.