Figure 2. Perturbing cellular Zn²⁺ causes global changes in chromatin accessibility.

(A) Volcano plots depicting genomic regions that exhibit a change in chromatin accessibility for cells treated with 50 μM TPA (left) or 30 μM ZnCl₂ (right). A positive log2FoldChange indicates an increase in accessibility, while a negative log2FoldChange indicates a decrease in accessibility. (B) DESeq2 differential accessibility analysis shows that most peaks are uniquely accessible depending on Zn²⁺ status. The overlap in the Venn diagrams indicates the peaks which are inversely accessible between the denoted treatments. (C) Genomic tracks showing the top hit for differential accessibilities for TPA (left) and ZnCl₂ (right) treated cells. Green boxes denote accessible chromatin as annotated using HMMRATAC.