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[Preprint]. 2023 Nov 21:2023.11.20.567954. [Version 1] doi: 10.1101/2023.11.20.567954

Figure 5. TP53 motifs show differential enrichment upon Zn2+ perturbation.

Figure 5.

(A) Barcode plots that show enrichment of the TP53 motif upon treatment of MCF10A cells with TPA (top). Addition of exogenous ZnCl2 results in depletion of the same motif (bottom). The barcode plots represent each quartile (Q1 – Q4) of the enrichment curves generated for the TP53 motif. Red indicates more enrichment of the motif; yellow is less enrichment of the motif.

(B) A subset of p53 binding sites from a ChIP-seq dataset (GSM3378513) are differentially accessible depending on cellular Zn2+ status. ATAC-seq reads were mapped to 2164 ChIP-seq peaks and subjected to differential accessibility analysis using DESeq2. With TPA treatment, 62 peaks showed significant (padj ≤ 0.1) changes in accessibility (51 decreased, 11 increased). With ZnCl2 treatment, 7 peaks showed significant changes in accessibility (1 increased, 5 decreased).