Figure 2:

A) Scatter plot of transcript fold-change expression with s-value < 0.01 (RNA-seq) in LPS-primed BMDMs treated, or not, with oxPAPC (100 μg ml⁻¹) for 3h (x-axis) or 18h (y-axis). Points correspond to genes. B) BMDMs were primed with LPS (1 μg ml⁻¹) and then treated, or not, with oxPAPC (100 μg ml⁻¹). Il10 mRNA levels were measured at the indicated time points by qPCR. n = 3, statistical significance was calculated using two-way ANOVA and Sidak’s multiple comparisons test. C-E) WT (n =16) and E06-scFv mice (n =10, isotype control n = 10, anti-IL10R) undergoing CLP were treated with anti-IL-10R antibody or isotype control, as indicated. Survival was followed over time. Kaplan–Meier curves with log-rank (Mantel-Cox) test are shown (C). (D) Body temperature loss was measured 8h after surgery (left) and bacteria loads in serum were analyzed 24h after CLP (right). Statistical significance was calculated using two-tailed t test. (E) WT and E06-scFv mice undergoing CLP were treated with anti-IL-10R antibody or isotype control, as indicated, and serum levels of IL-10, TNF, IL-1β and IL-6 were analyzed 8h after CLP. n =10 mice per group. Graph show means ± SD. Statistical significance was calculated using two-tailed t test.