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[Preprint]. 2024 Jun 11:2023.11.20.567799. Originally published 2023 Nov 20. [Version 2] doi: 10.1101/2023.11.20.567799

Figure 3. Reduction of nascent transcript levels after irradiation is mainly of the rDNA.

Figure 3.

A. Schematic of nascent transcript sequencing. B. Read counts for the total nascent transcripts, rDNA transcripts and protein coding transcripts before and 30 minutes after IR, normalized to ERCC spike in controls. C. Significantly changed nascent transcripts from protein coding genes are indicated upon irradiation, and the numbers indicate the number of upregulated and downregulated genes 30 minutes after IR. Data shown are an average of the two independent experimental repeats. D. Heat map of significantly increased and decreased nascent transcripts 30 minutes after IR, shown for two independent experimental repeats. Expression z-score was calculated by subtracting the overall average gene abundance from the raw expression for each gene and dividing that result by the standard deviation (SD) of all of the measured counts across all samples. E. Gene Ontology analysis of the top significantly enriched GO terms most upregulated after IR (pink) and most downregulated after IR (blue). Enriched gene number (red) and fold enrichment (blue) were showed in each GO term.