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[Preprint]. 2024 Jun 11:2023.11.20.567799. Originally published 2023 Nov 20. [Version 2] doi: 10.1101/2023.11.20.567799

Figure 6. CUL4B but not CUL4A contributes to transcriptional inhibition after irradiation.

Figure 6.

A. Analysis of CUL4A and CUL4B neddylation in U2OS cells after IR, in the absence of presence of 10 μM treatment for 3 hours with the neddylation inhibitor. N8 indicates the neddylated species. B. Analysis of nascent transcripts in Abl pre-B cell lines stably depleted of CUL4A or CUL4B 30 minutes after IR, as indicated. C. Short treatment of Abl pre-B cells with neddylation inhibitor MLN4924 is sufficient to block neddylation of CUL4B. D. The same cells used in C were analyzed for EU incorporation into nascent transcripts 30 minutes after irradiation, without or with the indicated time of MLN4924 treatment before irradiation.