Figure 3. Comparison of genetic determinants of P. aeruginosa colonization in the fly and mouse from previous studies.

(A) Alginate and psl polysaccharides, purines, pyrimidines, amino acids, cofactors, and respiration genes are critical for establishment of PAO1 and P. aeruginosa PA14 in invertebrate and vertebrate hosts, and at different body sites (14, 31). (B) Mutants in aceA, and pilM were positively selected across systems, in both PAO1 and PA14. Most of the genes encoding components of flagella and pili were positively selected in the mouse, but not the fly. Insertions in the mexEF-oprN operon, exsB, pagL, and bifA, among others, were overrepresented in the fly.