Figure 4. Validation of fitness determinants identified by Tn-Seq.

A 1:1 ratio of wild type PAO1 and the indicated transposon mutant was administered to flies for 24 hours. Flies were then given 5% sucrose for 48 hours and homogenized at 72 hours post-challenge. Bacteria were cultured on selective and non-selective media. Each symbol represents CFU recovered from individual fly, where n= 8 flies per group and one representative replicate is shown. Competitive Index = Log₁₀(CFU mutant/CFU wild type), where a competitive index of 0 indicates equal competitive fitness.