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Immunoblot analysis of E. coli XLOLR containing either pBK-CMV with a 3.2-kb insert of H. pylori genomic DNA (CP6) or pBK-CMV alone (CP1). Blots were probed with rabbit anti-H. pylori whole-cell antiserum and detected with alkaline phosphatase-conjugated anti-rabbit antibody. The numbers to the left indicate the sizes of the protein standards in kilodaltons. The arrow to the right indicates the 94-kDa antigen expressed by E. coli CP6.
