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[Preprint]. 2023 Nov 20:2023.11.20.567786. [Version 1] doi: 10.1101/2023.11.20.567786

Figure 2: Cry5B targets are present in Ascaris suum intestine:

Figure 2:

RT-PCR analysis of intestine (1i, 2i, 3i, 4i, 5i) and body wall (1m, 2m, 3m, 4m, 5m) of five separate female A. suum worms. Each lane represents the intestine or body wall of an individual worm. Asu-gapdh from the intestine (Ci) and body wall (Cm) was used as a positive control. N.C. = no template negative control. M = Fast Ruler Middle Range DNA Ladder (TermoFisher Scientific). A) Asu-bre-5 B) Asu-cdh-8. Note reduced intensity of bands highlighted by white circles in the body wall samples. All images are cropped. Images were taken under UV light with an exposure setting of 3 s per 1 frame. Original gel images are presented in Supplementary Fig. S1. C) Differential expression of Cry5B targets between intestine and body wall. Bar chart (expressed as mean ±SEM) demonstrating transcript level analysis from intestinal samples for bre-5 (grey bar) (5.24 ± 0.68) and cdh-8 (white bar) (6.86 ± 2.32) when compared to paired body wall samples. n = 15 individual worms over three biological replicates.