Skip to main content
[Preprint]. 2023 Nov 22:2023.11.22.568242. [Version 1] doi: 10.1101/2023.11.22.568242

Figure 2. ZYG-8 localizes diffusely across the oocyte spindle and is required for proper spindle assembly.

Figure 2.

(A) Schematic representation of the auxin-inducible degradation system for ZYG-8 depletion in C. elegans. (B) Immunofluorescence images of oocytes in the ZYG-8 AID strain showing ZYG-8 localization throughout meiosis; shown are tubulin (green), DNA (blue), ASPM-1 (red, left panel), and ZYG-8 (stained using a GFP antibody; red, right panel). ZYG-8 is diffusely localized to the oocyte spindle throughout Meiosis I and II. Due to diffuse ZYG-8 localization, ZYG-8 images shown are not deconvolved. (C) Immunofluorescence images of either control spindles or spindles treated overnight on auxin-containing plates (“long-term AID”); shown are tubulin (green), DNA (blue), ASPM-1 (red), and ZYG-8 (stained using a GFP antibody). (D-F) Quantification of spindle length, spindle angle, and number of ASPM-1-marked poles, demonstrating that long-term ZYG-8 depletion causes spindle defects. Scale bars = 5μm.