FIG. 12.

Single-cell RNA sequencing (scRNA-seq) analysis reveals non-monotonic cell-cycle dependence for multiple E. coli genes. scRNA-seq expression, converted to absolute mRNA copy-number, is plotted against the normalized cell age. Horizontal dotted lines indicate the expected steady-states levels before and after gene replication, demonstrating the failure of the simple mRNA-follows-dosage model. Blue line, fit to a model where gene replication is accompanied by a transient increase in promoter activity. Data from (Pountain et al., 2022). Additional analysis by Kevin McDonald and Tianyou Yao.