TABLE 1.

Function of virB2 to virB11 mutants in RSF1010 mobilization

Straina	Relevant characteristics	Mobilization of pML122ΔKmb
A348	Wild type	1.3 × 10−3
A348ΔB2	ΔvirB2	<6.3 × 10−7
A348ΔB3	ΔvirB3	<7.6 × 10−7
At12031	virB3::Tn5virB (2108)	<4.2 × 10−7
At12044	virB4::Tn5virB (3549)	<6.3 × 10−7
A348ΔB5	ΔvirB5	<8.8 × 10−7
A348ΔB6	ΔvirB6	<5.3 × 10−7
At12063	virB6::Tn5virB (6125)	<3.3 × 10−7
A348ΔB7	ΔvirB7	<7.1 × 10−7
A348ΔB8	ΔvirB8	<7.0 × 10−7
At12081	virB8::Tn5virB (6779)	<5.8 × 10−7
Ax42	virB9::Tn5virB (7878)	<1.0 × 10−6
A348ΔB10	ΔvirB10	<9.6 × 10−7
At12101	virB10::Tn5virB (8346)	<4.5 × 10−7
At12102	virB10::Tn5virB (8416)	<5.1 × 10−7
At10011	ΔvirB11	<1.1 × 10−6

^aConstruction of Tn5virB strains is described in the text, except for that of Ax42 (11), which was obtained from A. Binns. Numerical designation following Tn5virB refers to site of insertion according to the DNA sequence published by Ward et al. (26). All A348ΔB strains (4) were obtained from P. Christie. At10011 was from K. Stephens (24). 

^bConjugation assays were done as previously described (14). Frequencies of transfer of pML122ΔKm to recipient UIA143 are expressed as transconjugants per input donor. Data are the means of triplicates from a single assay. Three independent experiments were done, with similar results.