Extended Data Fig. 4 |. HLA-E expression levels on various cell types.
a, HLA-E expression levels on .221-SPE*01:03 cells, .221 cells pulsed with 100 μM VL9 peptides, PBMCs, and BLCLs. Error bars for PBMCs reflect variation across four donors (mean ± SD). The Y axis represents MFI obtained by 3D12 antibody staining minus MFI obtained by isotype control staining for each cell type. b, HLA-E expression levels on PBMCs and cell type subsets after 48 hours in culture with or without IFN-γ treatment. Data for two healthy donors (HD) are shown (HD75 and HD77) and represent triplicate experiments. Error bars represent the mean ± SD. P values for comparison between IFN-γ-treated and untreated cells were determined by a two-sided unpaired t-test: * - P < 0.05, ** - P < 0.01, *** - P < 0.001, **** - P < 0.0001.