Fig. 2 |. Jurkat^NKG2 reporter cells respond differentially to VL9-pulsed .221 and .221-SPE cells.

a, Schematic of the chimeric receptor design for expression in Jurkat reporter cells. Native receptors are shown on the left and chimeric reporter receptors are shown on the right. b, Reporter activity (percentage of CD69⁺ cells) of Jurkat^NKG2A and Jurkat^NKG2C cells after incubation with VL9-pulsed (100 μM peptide) or unpulsed (DMSO) .221 cells expressing endogenous E*01:01. c, Reporter activity of Jurkat^NKG2A and Jurkat^NKG2C cells after incubation with .221 target cells transduced with SPE*01:01, SPE*01:03 or vector control (vector ctrl). b,c, Data represent triplicate experiments. Data are mean ± s.d. ‘Target–’ designates reporter cell activity in the absence of target cells. P values were determined in comparison with DMSO (b) or vector control (c) using two-sided unpaired t-tests and labeled with asterisks if reporter activity showed a significant difference (P < 0.05) for VL9-pulsed (b) or both SPE*01:01- and SPE*01:03-transduced target cells (c). **P < 0.01, ***P < 0.001.