Figure 2. CD201 downregulation during emergency granulopoiesis is partially dependent on TLR4‐MyD88 signaling.

• A
  UMAP plot color‐coded for the expression of Procr.
• B
  Representative flow cytometry plots of BM cells isolated from mice treated with PBS control or LPS for 4 h. The x‐axes indicate CD201 expression. Numbers show percentage of CD201⁺ HSCs.
• C
  Quantification of panel b. The y‐axis indicates percentage of CD201⁺ HSCs in BM.
• D
  Absolute number of CD201⁺ HSCs in BM isolated from mice treated with PBS control or LPS for 4 h. The x‐axis indicates absolute number.
• E
  Representative flow cytometry plots indicating CD201 expression in HSCs isolated from WT and Cebpb KO mice treated with PBS or LPS as indicated for 4 h. Numbers indicate percentage of CD201⁺ HSCs.
• F, G
  Quantification of panel (E). Percentage of CD201⁺ HSCs (F) and CD201 mean fluorescence intensity (MFI) (G). X‐axes indicate fold change relative to PBS control mice (dashed lines).
• H
  Representative flow cytometry plots indicating CD201 expression in HSCs isolated from WT and MyD88 KO mice treated with PBS or LPS as indicated for 4 h. Numbers indicate percentage of CD201⁺ HSCs.
• I, J
  Quantification of panel (H). Percentage of CD201⁺ HSC (I) and CD201 MFI (J) in WT (gray columns) and MyD88 KO (pink column) mice treated with LPS for 4 h.
• K–M
  Quantification of MPP4 (K), MPP3 (L) and MPP2 (M) populations in WT (gray columns) and MyD88 KO (pink column) mice treated with LPS for 4 h. X‐axes indicate the fold change to PBS control.

Data information: Dashed lines indicate PBS levels. In this figure, data represent mean ± SD from at least two independent experiments. At least four animals were included in each group. Two‐tailed Student's t‐test was used to assess statistical significance (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, ns, not significant).

Source data are available online for this figure.