Fig. 1.

Depletion of α6-nAChRs in midbrain DA neurons by α6-miRNA. A Illustration showing the experimental framework wherein AAV-flex-mRuby2-α6miRNA was injected into the VTA of DAT-Cre mice 3 weeks prior to experimentation. B Fluorescent in situ hybridization images of neurons (DAPI; blue) from the VTA showing knockdown of Chrna6 (red; α6-nAChRs) with DAergic neurons in the midbrain identified by TH (green) and distinguished from GAD67 + neurons (cyan). C α6-miRNA treatment depleted α6-nAChRs in TH + neurons, but not α4 or β3 nAChRs