Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Timetable for HPLC method, including the calculated CVs
| Step | Solvent | Total runtime (min) | Flow rate (mL/min) | Volume |
|---|---|---|---|---|
| Injection | Sample | 0 | 16 μL | |
| Wash | Eluent A | 0–1.6 | 1.5 | 40 CV |
| Elution | Eluent B | 1.6–1.75 | 0–1.2 | 1.5 CV |
| 1.75–2.5 | 1.2 | 15 CV | ||
| Equilibration | Eluent A | 2.5–4.0 | 1.5 | 37.5 CV |
Heckel and colleagues present a fast HPLC-based affinity method to determine capsid titer and F/E ratio of rAAV vectors in gene therapy products to support analytics and process development.
