FIG. 2.

Time course of [³H]cPD1 binding. [³H]cPD1 binding to intact cells of the recipient strain OG1X and the donor strain OG1X carrying pAM351 is shown. Intact cells prepared from the bacteria growing at mid-log phase (OD₆₆₀ = 0.5) were resuspended at 1/10 of the original volume in fresh medium and incubated with 0.6 nM [³H]cPD1 at 37°C, and the radioactivity of the cells was then measured. The data represent average values from two incubations.