FIG. 5.

Equilibrium saturation binding (A) and Scatchard plot (B) of [³H]cPD1 binding to donor spheroplasts. The spheroplasts were prepared from OG1X carrying pAM351. The samples were run in duplicate. Specific binding (closed circles) was calculated by subtraction of the nonspecific binding (line [calculated from positions of closed triangles] determined for binding in the presence of a 1,000-fold molar excess of cold cPD1) from total binding (open circles).