Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
In silico restriction cloning of the codon-optimized multi-epitope subunit vaccine construct in the pET-21(+) vector between restriction sites (XhoI and BamH1). E. coli strain K12 can be used to express the final designed construct for vaccine manufacturing. The black circle shows the vector, and the red area denotes the vaccine construct.
