Table 3.
Selected CTL and HTL epitopes of the DENV1-4 serotypes.
| Protein | Serotype | CTL epitopes | MHC Alleles | IC50 | Antigenic Score | Allergenicity | Toxicity | PI | Mol.wt(Da) |
|---|---|---|---|---|---|---|---|---|---|
| EDIII | DENV1 | KLTLKGSYV | HLA-A*02:03 | 25.14 | 1.068(Antigenic) | Non allergen | Non toxin | 9.72 | 1008.36 |
| DENV2 | LTLKGSYVM | HLA-A*68:01 | 38.18 | 1.2035(Antigenic) | Non allergen | Non toxin | 8.94 | 1011.38 | |
| DENV3 | TILIKVEYK | HLA-A*03:01 | 38.81 | 1.7933(Antigenic) | Non allergen | Non toxin | 8.83 | 1106.51 | |
| DENV4 | VGSALTLHW | HLA-B*58:01 | 8.27 | 1.0554 (Antigenic) | Non allergen | Non toxin | 7.1 | 983.27 | |
| prM | DENV1 | SMAMRCVGI | HLA-A*02:06 | 39.69 | 1.5822(Antigenic) | Non allergen | Non toxin | 8.6 | 967.35 |
| DENV2 | RQEKGKSLLF | HLA-B*15:01 | 61.97 | 0.4506(Antigenic) | Non allergen | Non toxin | 10.01 | 1205.57 | |
| DENV3 | LTSRDGEPR | HLA-A*68:01 | 37.64 | 1.5125(Antigenic) | Non allergen | Non toxin | 6.42 | 1030.22 | |
| DENV4 | LLFKTTEGI | HLA-A*02:03 | 17.81 | 0.6686(Antigenic) | Non allergen | Non toxin | 6.35 | 1021.36 | |
| NS1 | DENV1 | LSMTCIAVGV | HLA-B*58:01, HLA-A*02:01 | 21.58 | 2.366(Antigenic) | Non allergen | Non toxin | 5.85 | 993.39 |
| DENV2 | AAEGINYAD | HLA-A*02:03, HLA-B*35:01 | 10.45 | 1.1155 (Antigenic) | Non allergen | Non toxin | 3.67 | 923.06 | |
| DENV3 | TAGPWHLGK | HLA-A*11:01 | 49.93 | 1.1355(Antigenic) | Non allergen | Non toxin | 9.11 | 966.24 | |
| DENV4 | TQTGPWHLGK | HLA-A*31:01 | 41.23 | 1.1272 (Antigenic) | Non allergen | Non toxin | 9.11 | 1124.42 | |
| Protein | Serotype | HTL epitopes | MHC allele | IC50 | Antigenic Score | Allergenicity | Toxicity | PI | Mol.wt(Da) |
| EDIII | DENV1 | ALTGATEIQSGTTTI | HLA-DRB1*07:01 | 34.5 | 0.7846(Antigenic) | Non allergen | Non toxin | 4 | 1463.8 |
| DENV2 | GSYVMCTGSFKLEKE | HLA-DRB3*01:01 | 36.8 | 0.8936(Antigenic) | Non allergen | Non toxin | 6.45 | 1679.15 | |
| DENV3 | SNIVIGGDALKINWY | HLA-DRB1*13:02 | 21.4 | 0.9058(Antigenic) | Non allergen | Non toxin | 9.65 | 1663.14 | |
| DENV4 | EIRDVNKEKVVGRII | HLA-.DRB1*07:01 | 79.2 | 0.6148(Antigenic) | Non allergen | Non toxin | 8.93 | 1768.32 | |
| prM | DENV1 | LMLVTPSMAMRCVGI | HLA-.DRB1*01:01 | 39.37 | 1.0701(Antigenic) | Non allergen | Non toxin | 10.11 | 1666.15 |
| DENV2 | KSLLFKTGNMCTLMA | HLA-DRB1*01:01 | 51.4 | 0.47 (Antigenic) | Non allergen | Non toxin | 7.09 | 1632.03 | |
| DENV3 | MCDDTVTYK | HLA-DRB1*03:01 | 1.7 | 0.77(Antigenic) | Non allergen | Non toxin | 8.84 | 1689.21 | |
| DENV4 | DQKAVHADMGYWIES | HLA-DRB1*03:01 | 20.5 | 0.6105 (Antigenic) | Non allergen | Non toxin | 4.54 | 1750.13 | |
| NS1 | DENV1 | LTWLGLNSRSTSLSM | HLA-DRB3*02:02 | 31.6 | 1.9214 (Antigenic) | Non allergen | Non toxin | 10.11 | 1666.15 |
| DENV2 | AAIKDNRAVHADMGY | HLA-DRB5*01:01, HLA-DRB1*15:01 | 17.24 | 1.1778 (Antigenic) | Non allergen | Non toxin | 7.09 | 1632.03 | |
| DENV3 | GSWKLEKASLIEVKT | HLA-DRB1*01:01 | 9.4 | 0.9481(Antigenic) | Non allergen | Non toxin | 8.84 | 1689.21 | |
| DENV4 | DQKAVHADMGYWIES | HLA-DRB3*01:01 | 69.4 | 0.6105 (Antigenic) | Non allergen | Non toxin | 4.54 | 1750.13 |
*The antigenicity score was provided by VaxiJen. An antigenic score >0.4 indicates that all the epitopes are antigenic in nature. Some of the epitopes we considered in the final vaccine construct are antigenic in nature but have an IC50 >50 (represented in bold). The chosen epitopes are non-allergenic and non-toxic.