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. 2023 Nov 16;71(47):18478–18488. doi: 10.1021/acs.jafc.3c04503

Table 2. Caffeine Content and Acute Toxicity Assessment with Daphnia magna of the Coffee Cell Culture and Coffee Bean Samples.

      EC50 values [mg/L]
samples caffeine [mg/g] pH 24 h 48 h
lyophilized coffee cells 0.22 6.96 >1000 >1000
lyophilized and roasted coffee cells 0.22 6.81 >1000 >1000
roasted Arabica beans 8.59 6.96 930 680
K2Cr2O7 (negative control)     0.83 0.93