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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: J Vis Exp. 2023 Feb 17;(192):10.3791/64879. doi: 10.3791/64879

Figure 1: Setup of gross dissection and heart cannulation area.

Figure 1:

From right to left: a. The anesthesia induction chamber for the animal is located near the dissection area. b. A snorkel for optional volatile gas scavenger. c. A dissection pad, where the animal will be placed supine, is flanked by (clockwise) d. a nosecone, to provide continued anesthesia to the rodent. e. Hemostats, f. surgical scissors, and g. curved fine scissors placed for easy access with the dominant (cutting) hand. h. Curved iris forceps placed for easy access with the non-dominant hand. Upper limbs of the rodent may be affixed to the dissection pad using tape. i. A dissection dish (or small beaker) with perfusion solution should be placed nearby to rinse the heart. j. An area staged for cannulation must be placed nearby. A syringe with an appropriate cannula is mounted on a ring stand. k. (Inset) A closer image of a 16 G cannula, with 1 mm of PE205 tubing attached and a suture loosely knotted.