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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: J Vis Exp. 2023 Feb 17;(192):10.3791/64879. doi: 10.3791/64879

Figure 2: Experimental area and tools.

Figure 2:

(A) Experimental area. The experimental chamber and data acquisition system are prepared on a nearby inverted microscope (left). Trabecula isolation and mounting occurs under a stereoscope (right). (B) Tips of two forceps, large and small Vannas scissors, and a custom glass probe are prepared by soaking in 10% BSA solution. (C) Additional dissection tools. A silicone elastomer-plated dish allows for mounting the heart during fine dissection. A 7 mL transfer pipette with the end cut is shown below the dish and glass probe. The cut tip of the transfer pipette is discarded, and an enlarged bore is used to transfer the muscle, with minimal risk of stretching or dehydration. (D) Magnified image of the glass pipette, which is approximately 2 mm long and 0.25–0.5 mm in diameter.