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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: J Vis Exp. 2023 Feb 17;(192):10.3791/64879. doi: 10.3791/64879

Figure 4: Representative traces and results.

Figure 4:

(A) A single cardiac trabecula illuminated using a gooseneck LED light at a steep (~75°) angle from the axis of the microscope lens, which enhances contrast of the trabecula. In this example, two images are tiled to show the full length of the trabecula. (B) Stress-time (top) and strain-time (bottom) curves for the same trabecula. An isometric twitch, along with three load-clamp twitches at increasing end systolic strain rates are shown. (C) Representative MCR calculation. MCR is defined as the slope of the line between the relaxation rate and strain rate. As noted in the discussion, strain rates may be restricted to provide a quantitative, reproducible MCR.